ICSI procedure step by step timeline?

Dr.Anjali Pathak
05 August 2025

ICSI Step‑by‑Step Timeline
1. Consultation & Testing

• Initial evaluation, lab tests, scan planning — takes up to 1–2 weeks depending on protocol

• Daily hormone injections (FSH, LH) for ~10–14 days to grow multiple eggs
• Monitored via bloodwork and ultrasound

• Trigger shot given when follicles ripen, followed 34–36 hours later by egg collection under sedation (10–15 minutes) The Hewitt Fertility Centre

• Same day: sperm sample or surgical extraction
• Embryologist injects one selected sperm per egg under a microscope
• Fertilization checked next morning (~16–18 h later) https://cryobankamerica.com/icsi-procedure/?utm_source=chatgpt.com

• Embryos monitored for 3–5 days until Day 3 (6–8 cells) or Day 5 (blastocyst) Institute for Reproductive Health+1

• Selected embryo placed in uterus via catheter under ultrasound—usually on Day 3 or Day 5 (takes about 10–15 minutes)

• Progesterone starts after transfer, continued until around 10–14 days later when pregnancy test is taken

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Dr. Nandani Priya
08 August 2025

ICSI stands for Intracytoplasmic Sperm Injection. Here’s a clear, easy timeline:

1. Ovarian stimulation: daily hormone shots for ~8–12 days to mature multiple eggs.

2. Egg retrieval: short procedure under mild anaesthesia to collect eggs.

3. Sperm prep: sperm is collected/sorted the same day.

4. ICSI in the lab: a single sperm is injected into each mature egg.

5. Fertilization check: about 16–18 hours later (day 1) embryologists check for fertilized eggs.

6. Embryo culture: embryos grow in the lab for 2–5 days.

7. Embryo transfer: one or more embryos are placed into the uterus (commonly day 2/3 or day 5).

8. Pregnancy test: done ~10–14 days after transfer; medicines (luteal support) are usually continued until then.

Official info: NHS  https://www.uhcw.nhs.uk/ivf/treatments/icsi/

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